Accurate and Reliable Multi Chamber Pcr Chip with Sample Loading and Primer Mixing Using Vaccum Jackets for N X M Quantitative Analysis

This paper reports a high-throughput n × m well array PCR chip with an accurate and reliable method for sample loading and primers mixing. PCR mixture could be precisely introduced into each reaction chamber, which was prespotted and dried up with each primer set, by sucking the air through PDMS gas permeable wall. In this approach, the mixing of primer and sample at constant ratio was achieved and an equal amplification rate at an unique condition was ensured across the reaction chambers. Our method can be used to develop multi-target sample amplification chips for diagnostic applications.